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KMID : 1098420050130060278
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Korean Journal of Medicinal Crop Science
2005 Volume.13 No. 6 p.278 ~ p.286
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Classification of Artemisia spp. Collections Based on Morphological Characters and RAPD Analysis
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Park Sang-Kyu
Chung Bong-Hwan
Kim Hong-Sig
Cho Yong-Gu
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Abstract
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This study was conducted to classify 80 Artemisia spp. collections based on morphological characters and RAPD analysis to get the basic information of Artemisia spp. collections as medicinal plants. The eighty Artemisia spp. collections were classified into 5 groups with the maximum distance 0.82 between clusters based on the complete linkage cluster analysis with morphological traits. Out of 80 operon primer, 10 primers showing polymorphic bands were selected for RAPD analysis. Among the 98 bands amplified with the primers, 68 (69%) bands showed polymorphism. The number of amplified bands ranged from 8 to 10 with an average number of 9.8 bands. Artemisia spp. collections classified into 6 groups with the similarity value of 0.63 in dendrogram derived from the cluster analysis based on RAPDs. Group consisted of 29 collections. Group, which is the largest one, contained 40 collections. Most of the A. asiatica and A. feddei LEV et VNT. were classified into Group and. The rest of the collections (31%) were classified into Group III{sim}V.
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KEYWORD
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Artemisia, mugwort, morphological characters, RAPD, similarity value, phylogenetic
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